In the anaerobic zone, polyphosphate-accumulating microorganisms take up volatile fatty acids and release phosphorus; then in the oxic zone, phosphorus is retained in the biomass, providing Enhanced Biological Phosphorus Removal. Nitrogen removal is limited because there is no anoxic stage; therefore, in plants with Total Nitrogen targets, either transition to chemical phosphorus + Modified Ludzack–Ettinger process or addition of an anoxic stage is preferred.